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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
published: 18 Sep 2013 last updated: 18 Feb 2021
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Glyphosate tolerant canola
EN
61061
Yes
DP-Ø61Ø61-7
The canola (Brassica rapa) was modified for tolerance to the glyphosate herbicide through the insertion of the Bacillus licheniformis glyphosate-N-acteyltransferase gene, which inactivates glyphosate through acetylation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
PHP28181
EN
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-104802-5 Polyubiquitin10 gene promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-48363-4 Glyphosate-N-Acteyltransferase gene | Bacillus licheniformis (BACLI)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-100367-4 Proteinase inhibitor II gene terminator | Solanum tuberosum (Potato, SOLTU)
    Terminator
The canola contains one gene cassette for Bacillus licheniformis glyphosate-N-ccteyltransferase (gat). The coding sequence was under control of an Arabidopsis thaliana ubiquitin-10 promoter and a Solanum tuberonsum proteinase inhibitor II 3' untranslated region.

The gat coding sequence expresses the GAT4621 version of the protein, which was functionally improved by a gene shuffling process to optimize the kinetics of glyphosate acetyltransferase.

Southern blot analysis indicated that a single, intact PHP28181 DNA fragment was inserted into the genome with no plasmid backbone DNA.
EN
LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
See patent in additional information section below
EN
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 2
Country's Decision or any other Communication LMO identification 1
Living Modified Organism Related LMO(s) 1